The Johnston lab at San José State University consists of a team of gene therapists with the ambition of finding a cure for blood disorders. Specifically, we are exploring the use of the CRISPR-Cas9 system to enhance the endogenous repair pathway of homologous recombination. In this manner, we can incorporate missing transgenes into specific locations in the genome of hematopoietic stem cells. With this strategy we hope to change the prognosis of numerous individuals across the globe.
The Johnston lab prioritizes the cultivation of an inclusive and collaborative environment in which lab members are empowered to freely and openly share their different views, ideas, and experiences. We strongly believe that bringing together individuals with diverse backgrounds increases the chances of uncovering new and exciting principals in the biomedical sciences. As a lab, we recognize that institutionalized discrimination -- based on race, gender, disability, age, socioeconomic status, citizenship and immigration status among other attributes -- persists in the academic community. We acknowledge multiple forms of discrimination and the inherent value of diversity. We are committed to fostering diversity within avenues of our work through cultivating antiracist and equity-minded approaches.
INNOVATIVE SAFE HARBORS
The RhD locus is disrupted in a substantial portion of the general population. This gene locus encodes for the RhD blood antigen. Individuals in which the RhD locus is disrupted are clinically classified to have the Rh-negative blood type. No phenotypic consequence has yet to be associated with the loss of the RhD gene; therefore this location in the genome is prime for gene addition. These studies will confirm the utilization of the RhD locus as a safe harbor extending the application of this study beyond hemophilia A.
IDLV DONOR DELIVERY
Viral delivery vectors are ideal for gene delivery having the ability to transduce both dividing and non-dividing cells. However, only integrating lentiviral vectors have enough carrying capacity to contain a large transgene as FVIII. Yet, lentiviral vectors integrate in an uncontrollable manner and can cause genomic instability. The integrase deficient lentiviral vector, on the other hand, does not contain the gene for integrase, the enzyme that allows for genomic incorporation of viral DNA. Therefore, IDLV offers the newest means to enhance gene editing. Because IDLVs are based on lentiviruses, they can transfer large genetic loads efficiently and transduce a large variety of dividing and non-dividing cells, all without disrupting the integrity of the genome.
PLATELET EXPRESSION OF FVIII
As an active locus in the hematopoietic system, the vWF locus is prime for FVIII expression. In addition, the expression of FVIII from the vWF locus provides a treatment option for the substantial portion of hemophilia patients that have developed inhibitors to recombinant FVIII. vWF is contained within α granules until platelets become activated. This unique expression characteristic would prevent FVIII from being accessible to inhibitors, which circulate in the bloodstream, until blood vessels rupture causing platelets to activate. Therefore, FVIII would be safely sequestered in the α granules of platelets until physiologically necessary.